Navigating the High-Content Imaging Process

High-content imaging is a rapidly growing field that has shown great potential in all phases of the drug discovery process.1 Recently, high-content imaging has expanded to become a more widely used basic research tool. At the same time, applications of high-content imaging have broadened in scope, showing utility in systems biology, siRNA screening, and in other functional genomics and proteomics approaches. Whether one has extensive experience or is a novice in the field, navigating through the high-content imaging process is a complex task. A thorough understanding of the basic concepts, strategies, and goals of high-content imaging is crucial to ensure efficient and successful implementation of the technology. This application note examines some of the more important areas that should be considered when developing and optimizing a highcontent imaging application.